Processes for preparing acarviosyl transferase and for using...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C12N 9/10 (2006.01) C12P 19/18 (2006.01) C12P 19/26 (2006.01)

Patent

CA 2200421

The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expression of the acarviosyl transferase in a heterologous host organism, to the use of the acarviosyl transferase for converting acarbose minor constituents into acarbose or for preparing acarbose homologues, to the use of the acarviosyl transferase in acarbose purification, and also to the preparation of production mutants in which formation of minor constituents is reduced by means of inactivation of the acarviosyl transferase gene.

Acarviosyl-transférase provenant d'actinomycètes, principalement d'Actinoplanes sp. SE 50/110 et de ses mutants; méthode pour isoler, purifier et caractériser l'enzyme; méthode pour isoler et caractériser le gène acbD codant l'acarviosyl-transférase; expression de l'acarviosyl-transférase chez un organisme hôte hétérologue; utilisation de l'acarviosyl-transférase pour la conversion de constituants mineurs de l'acarbose en acarbose ou pour la préparation d'homologues de l'acarbose; utilisation de l'acarviosyl-transférase dans la purification de l'acarbose; préparation de mutants de production, où la formation de constituants mineurs est réduite par inactivation du gène de l'acarviosyl-transférase.

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