Processes for producing cmp-n-acetylneuraminic acid

Details Processes for producing cmp-n-acetylneuraminic acid Processes for producing cmp-n-acetylneuraminic acid

C - Chemistry, Metallurgy

12

P

C12P 19/26 (2006.01) C12N 9/12 (2006.01) C12N 9/88 (2006.01) C12N 9/90 (2006.01) C12N 15/52 (2006.01) C12P 19/30 (2006.01)

Patent

CA 2492838

A process for producing CMP-N-acetylneuraminic acid (CMP-NeuAc) characterized in that yeast cells, N-acetylglucosamine-6-phosphate 2-epimerase (GlcNAc-6P 2- epimerase), N-acetylneuraminate lyase (NeuAc lyase) and CMP-N- acetylneuraminate synthase (CMP-NeuAc synthase) are added to a reaction system containing N-acetylglucosamine (GlcNAc), pyruvic acid and cytidine-5~- monophosphate (CMP) and reacted. Another process for producing CMP-NeuAc characterized in that yeast cells, GlcNAc-6P 2-epimerase, NeuAc synthase and CMP-NeuAc synthase are added to a reaction system containing GlcNAc and CMP and reacted.

Cette invention se rapporte à un procédé servant à produire de l'acide CMP-N-acétylneuraminique (CMP-NeuAc) se caractérisant en ce que des cellules de levure, de la N-acétylglucosamine-6-phosphate 2-épimérase (GlcNAc-6P 2-épimérase), de la N-acétylneuraminate lyase (NeuAc lyase) et de la CMP-N-acétylneuraminate synthase (CMP-NeuAc synthase) sont ajoutées à un système de réaction contenant de la N-acétylglucosamine (GlcNAc), de l'acide pyruvique et du cytidine-5'-monophosphate (CMP) et mises en réaction. Un autre procédé servant à produire de l'acide CMP-NeuAc se caractérise en ce que des cellules de levure, de la GlcNAc-6-P 2-épimérase, de la NeuAc synthase et de la CMP-NeuAc synthase sont ajoutées à un système de réaction contenant de la GlcNAc et du CMP et mises en réaction.

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