Processes for the preparation and separation of macromolecules

B - Operations – Transporting – 01 – D

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204/99, 195/1.12

B01D 57/02 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 27/447 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2012379

A process for the electrophoretic separation of charged macromolecules which comprises applying to said macromolecules a periodic sequence of pulses with each period comprising a multiplicity of electric field pulses of negative and positive polarities wherein the negative polarity pulses are applied for a longer total time duration than the positive polarity pulses within each period, and the average intensity of the negative polarity pulses is less than the average intensity of the positive polarity pulses.

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