C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.17, 195/1.
C12N 15/00 (2006.01) C12N 1/20 (2006.01) C12N 15/74 (2006.01) C12P 21/02 (2006.01)
Patent
CA 1270216
Abstract of the disclosure: A hybrid plasmid with the replicon inherent to the methylotrophic bacterium is obtained from a plasmid from obligate methylotrophic bacteria, for example Me- thylomonas, on the one hand and a plasmid with selec- tion markers on the other hand. This hybrid plasmid is introduced by transformation into a host organism such as E. coli, if appropriate after previous introduction of further genetic information. After amplification and appropriate selection, the clones are treated with a suitable conjugative plasmid, and the mobilizability de- fect is abolished by introduction of a further suitable plasmid. The clones thus obtained are conjugated with, preferably plasmid-free, obligate methylotrophic bacte- ria as recipient, clones being obtained, after selec- tion, which express proteins corresponding to the gene- tic information introduced. It is also possible to pre- pare spheroplasts from the bacterium serving as the re- cipient and to insert the hybrid plasmids into the for- mer by transformation.
430123
Esser Karl
Marquardt Rudiger
Prave Paul
Stahl Ulf
Winnacker Ernst L.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Esser Karl
Hoechst Aktiengesellschaft
Marquardt Rudiger
Prave Paul
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