Processing of plasmid-containing fluids

Details Processing of plasmid-containing fluids Processing of plasmid-containing fluids

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2410184

Disclosed is a method for processing a plasmid-containing fluid comprising contacting the plasmid-containing fluid with two charged membranes and obtaining a plasmid-enriched fluid such as a purified plasmid. In an embodiment, the first charged membrane is positively charged, and the second charged membranes is positively or negatively charged membrane. In a preferred embodiment, both first and second charged membranes are positively charged. In another embodiment, the plasmid-containing fluid is contacted with two positively charged membranes and a third charged membrane. The present invention further provides a system and a kit for processing plasmid- containing fluids.

La présente invention concerne un procédé de traitement d'un fluide contenant des plasmides, consistant à mettre en contact ledit fluide avec deux membranes chargées et à obtenir un fluide enrichi en plasmides, par exemple, un fluide à base de plasmides purifiés. Dans un premier mode de réalisation, la première membrane est chargée positivement, et la seconde membrane est chargée positivement ou négativement. Dans un autre mode de réalisation, les première et seconde membranes sont chargées positivement. Dans un autre mode de réalisation encore, le fluide contenant des plasmides est mis en contact avec deux membranes chargées positivement et une troisième membrane chargée. La présente invention concerne également un système et un kit de traitement de fluides contenant des plasmides.

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