Processing polynucleotide-containing samples

Details Processing polynucleotide-containing samples Processing polynucleotide-containing samples

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12M 1/34 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2565572

Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.

Il est exposé des procédés et des systèmes servant à traiter des polynucléotides (par exemple de l'ADN). Une région de traitement comprend une ou plusieurs surfaces (par exemple des surfaces de particules) modifiées avec des ligands qui retiennent des polynucléotides sous un premier ensemble de conditions (par exemple de température et de pH) et libèrent les polynucléotides sous un deuxième ensemble de conditions (par exemple une température plus élevée et/ou un pH plus basique). On peut utiliser la région de traitement, par exemple, pour concentrer des polynucléotides d'un échantillon et/ou séparer des inhibiteurs de réactions d'amplification des polynucléotides. Il est exposé des dispositifs microfluidiques ayant une région de traitement.

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