Producing pullulanase with organisms having a superior...

Details Producing pullulanase with organisms having a superior... Producing pullulanase with organisms having a superior...

C - Chemistry, Metallurgy

12

P

195/123, 195/138

C12P 21/00 (2006.01) C12N 9/44 (2006.01)

Patent

CA 1047424

ABSTRACT OF THE DISCLOSURE Improved pullulanase yields are achieved by incubating culture mediums containing pullulanase producing mutants of the Klebsiella genus. The mutants generally produce approximately equivalent amounts of extracellular and superficially bound pullulanase in an easily recoverable and usable form. Conventional pullulanase inducers such as maltose, maltotriose and/or pullulan repress the mutant strains capacity to produce pullulanase. The mutants are capable of elaborating pullulanase in a culture media containing dextrose as the sole carbohydrate source.

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