C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C12N 1/13 (2006.01) C12N 9/88 (2006.01) C12N 15/31 (2006.01) C12N 15/79 (2006.01)
Patent
CA 2443908
Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, .alpha.-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.
La présente invention concerne des séquences d'acides nucléiques et d'acides aminés pour la synthase d'acétolactate, les régions de régulation de synthase d'acétolactate, le promoteur .alpha.-tubuline, un promoteur dérivé du système de la synthase du polykétide de Thraustochytriales, et un promoteur de la désaturase d'acide gras, chacun dérivé d'un micro-organisme de Thraustochytriales. L'invention concerne également des vecteurs recombinants utiles pour la transformation de micro-organismes de Thraustochytriales. Le molécules d'acides nucléiques recombinantes de la présente invention peuvent être utilisées pour l'expression d'acides nucléiques étrangers dans un micro-organisme de Thraustochytriales ainsi que pour la délétion, la mutation, ou l'inactivation de gènes dans des micro-organismes de Thraustochytriales.
Matthews T. Dave
Metz James G.
Ramseier Tom M.
Roessler Paul G.
Martek Biosciences Corporation
Mbm Intellectual Property Law Llp
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