Production and purification of il-29

Details Production and purification of il-29 Production and purification of il-29

C - Chemistry, Metallurgy

07

K

C07K 14/54 (2006.01)

Patent

CA 2625208

The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.

La présente invention concerne des vecteurs d'expression et des procédés utilisant un système d'expression dans E. coli pour une production à grande échelle de IL-29. Les vecteurs utilisent la séquence codante de IL-29 avec des modifications spécifiques de certains nucléotides, destinées à optimiser les codons et la structure secondaire de l'ARNm pour une traduction dans E. coli. L'invention concerne également des procédés de production, de purification et de traitement au PEG d'un polypeptide IL-29.

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