Production and screening process for phytochemical...

C - Chemistry – Metallurgy – 12 – P

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C12P 17/02 (2006.01) A61K 31/337 (2006.01) C07D 305/14 (2006.01) C12N 5/00 (2006.01) C12P 1/00 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2242929

A process for producing phytochemicals from plant cell cultures is provided. That process includes the step of culturing plant cells that produce a particular phytochemical in a multi-phase culture medium that includes an aqueous phase and a hydrophobic phase having a high partition coefficient for paclitaxel relative to the aqueous phase. The phytochemical is recovered from the hydrophobic phase. A process of screening plant cell cultures to plant cell lines that produce a particular phytochemical using the production process is also provided. A preferred phytochemical is paclitaxel.

L'invention concerne un procédé permettant de produire des substances phytochimiques à partir de cultures de cellules végétales. Le procédé comprend les étapes suivantes: on cultive des cellules végétales produisant une substance phytochimique particulière dans un milieu de culture multiphasique, qui comprend une phase aqueuse et une phase hydrophobe ayant un coefficient de répartition eau/huile élevé pour le paclitaxel par rapport à la phase aqueuse; puis on récupère la substance phytochimique dans la phase hydrophobe. L'invention concerne également un procédé permettant d'analyser des cultures de cellules végétales pour sélectionner des souches produisant une substance phytochimique particulière à l'aide dudit procédé de production. Un produit phytochimique préféré est la paclitaxel.

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