C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/81 (2006.01) C12N 15/62 (2006.01)
Patent
CA 2058116
ABSTRACT OF THE DISCLOSURE A cDNA copy of the M2 dsRNA encoding the K2 killer toxin of Saccharomyces cerevisiae was expressed in yeast using the yeast ADH1 promoter. This construct produced K2-specific killing and immunity functions. Efficient K2-specific killing and immunity functions. Efficient K2-specific killing was dependent on the action of the KEX2 endopeptidase and KEX1 carboxypeptidase, while K2-specific immunity was independent of these proteases. comparison of the K2 toxin sequence with that of the K1 toxin sequence shows that although they share a common processing pathway and are both encoded by cytoplasmic dsRNAs of similar basic structure, the two toxins are very different at the primary sequence level. Site-specific mutagenesis of the cDNA gene establishes that one of the two potential KEX2 cleavage sites is critical for toxin action but not for immunity. Immunity was reduced by an insertion of two amino acids in the hydrophobic amino- terminal region which left toxin activity intact, indicating an independence of toxin action and immunity.
Dignard Daniel
Germain Doris
Tessier Daniel C.
Thomas David Y.
Whiteway Malcolm
Anderson J. Wayne
As Represented By The Ministe Minister Of The National Research Her Majesty The Queen In Right Of Canada
Dignard Daniel
Tessier Daniel C.
Thomas David Y.
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