Production and use of epitope-tagged hepatitis c virus particle

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/09 (2006.01) A61K 39/29 (2006.01) A61P 1/16 (2006.01) A61P 31/12 (2006.01) C07K 16/08 (2006.01) C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12N 7/02 (2006.01)

Patent

CA 2696437

This invention relates to a nucleic acid comprising a nucleic acid sequence encoding an epitope tag peptide in the hypervariable region 1 of the E2 protein of naturally occurring or chimeric hepatitis C virus (HCV) genome, an epitope- tagged HCV particle encoded by such nucleic acid, and a method for purifying the HCV particles at a high purity in a simple manner with the use of an anti-epitope tag antibody- immobilized support.

L'invention porte sur un acide nucléique ayant une séquence nucléotidique codant un peptide marqueur épitope au niveau d'une région hypervariable 1 dans la protéine E2 2 sur un génome du virus de l'hépatite C (VHC) naturel ou chimérique ; sur des particules du VHC marquées par un épitope codées par l'acide nucléique ; et sur un procédé de purification de particules du VHC d'une manière simple et à une pureté élevée par l'utilisation d'un support ayant un anticorps marqueur anti-épitope immobilisé sur celui-ci.

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