Production and use of normalized dna libraries

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 40/08 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C40B 50/00 (2006.01) C40B 50/06 (2006.01)

Patent

CA 2258175

Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.

L'invention concerne un procédé permettant de former une banque normalisée d'ADN génomiques sur la base d'un échantillon de l'environnement; ledit procédé consiste: (a) à isoler une population d'ADN génomiques à partir d'un échantillon de l'environnement; (b) à analyser la complexité de la population d'ADN génomiques ainsi obtenue; (c) (i) à augmenter le nombre de copies des populations d'ADN ainsi isolées et/ou (ii) à récupérer une fraction de l'ADN génomiqiue isolé qui possède les caractéristiques désirées; et (d) à normaliser la représentation de divers ADN à l'intérieur d'une population d'ADN génomiques, de manière à créer une banque normalisée d'ADN génomiques sur la base de l'échantillon de l'environnement. L'invention se rapporte également à une banque normalisée d'ADN génomiques, formée sur la base d'un échantillon de l'environnement au moyen du procédé décrit.

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