Production and use of yeast having increased cellulose...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/19 (2006.01) C12N 15/09 (2006.01) C12P 7/08 (2006.01)

Patent

CA 2736975

The present invention provides a method for producing an yeast having an increased cellulose hydrolysis ability. The method includes the step of introducing increased integration copy numbers of both a gene for an enzyme capable of hydrolyzing crystalline cellulose and a gene for an enzyme capable of hydrolyzing noncrystalline cellulose into a noncellulolytic yeast to give a transformed yeast. The yeast having an increased cellulose hydrolysis ability can be suitably used for ethanol production from cellulose-based materials.

La présente invention concerne un procédé de production dune levure présentant une augmentation de la capacité dhydrolyse de la cellulose. Le procédé comprend une étape consistant à introduire à la fois un nombre accru de copies dintégration dun gène pour une enzyme capable dhydrolyser une cellulose cristalline et un nombre accru de copies dintégration dun gène pour une enzyme capable dhydrolyser une cellulose non cristalline dans une levure nhydrolysant pas la cellulose pour produire une levure transformée. La levure présentant une augmentation de la capacité dhydrolyse de la cellulose peut être utilisée de manière appropriée pour la production déthanol à partir dune substance cellulosique.

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