Production of 1,3-propanediol from glycerol by recombinant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/60 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12N 9/88 (2006.01) C12P 7/18 (2006.01)

Patent

CA 2220441

A process is provided for the bioconversion of glycerol to 1,3-propanediol in which genes from a bacteria known to possess a diol dehydratase enzyme for 1,2- propanediol degradation are cloned into a bacterial host and the host is grown in the presence of glycerol; expression of the foreign genes in the host cell facilitates the enzymatic conversion of glycerol to 1,3-propanediol which is isolated from the culture.

L'invention se rapporte à un procédé de bioconversion de glycérol en 1,3-propanediol dans lequel des gènes, issus de bactéries connues pour posséder une enzyme diol déshydratase favorisant la dégradation en 1,2-propanediol, sont clonés dans un hôte bactérien, et l'hôte se développe en présence de glycérol; l'expression des gènes étrangers dans la cellule hôte facilite la conversion enzymatique du glycérol en 1,3-propanediol qui est isolé du milieu de culture.

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