C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/131, 195/68
C12P 21/00 (2006.01) C07H 7/027 (2006.01) C12P 7/40 (2006.01) C12P 7/58 (2006.01) C12P 7/60 (2006.01)
Patent
CA 1043283
PROCESS FOR PREPARING 2-KETO-L-GULONIC ACID ABSTRACT 2-Keto-L-gulonic acid is prepared directly from D-glucose by microbial conversion utilizing mixed culturing on or mixed con- tacting with a medium containing D-glucose, employing at least two kind of microorganisms; 2,5-diketo-D-gluconic acid producing strains which belong to the genera of Acetobacter, Acetomonas and Gluconobacter and strains capable of converting the 2,5-diketo-D- gluconic acid into 2-keto-L-gulonic acid which belong to the genera of Brevibacterium and Corynebacterium. Both the incubation of the microorganisms in a medium containing D-glucose and the direct contact of any products obtained from the cells of the microorganisms with the substrate may be used in the disclosed process. By-production of the undesired optical isomer, 2-keto- D-gluconic acid, of the intended product is effectively prevented by employing the mixed culturing or contacting because of the presence of the 2,5-diketo-D-gluconic acid producing strain or any products thereof in the medium during at least part of the entire process.
228404
Honjo Tahiko
Kageyama Bunji
Kobayashi Kobee
Sonoyama Takayasu
Tani Hiroyoshi
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