Production of a lipid acyltransferase from transformed...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/18 (2006.01) C12N 1/21 (2006.01) C12N 15/75 (2006.01)

Patent

CA 2673954

The present invention relates to a method for the production of a lipid acyltransferase comprising the steps of: (i) providing a Bacillus licheniformis cell; (ii) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding a lipid acyltransferase; and (iii) expressing the lipid acyltransferase in the cell under the control of a promoter sequence. In addition, the present invention further relates to the use of Bacillus licheniformis to express a lipid acyltransferase, a Bacillus licheniformis host cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous to B. licheniformis.

La présente invention concerne une méthode de production d'une lipide acyltransférase comprenant les étapes suivantes : (i) la mise à disposition d'une cellule de bacillus licheniformis ; (ii) la transformation de la cellule de bacillus licheniformis par une séquence nucléotide hétérologue encodant une lipide acyltransférase ; et (iii) l'expression de la lipide acyltransférase au sein de la cellule sous le contrôle d'une séquence promotrice. De plus, la présente invention concerne également l'utilisation de bacillus licheniformis dans l'expression d'une lipide acyltransférase, une cellule hôte de bacillus licheniformis comprenant une lipide acyltransférase hétérologue et un vecteur comprenant une séquence nucléotide encodant une lipide acyltransférase liée de façon fonctionnelle à une séquence promotrice homologue de B. licheniformis.

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