Production of a multimeric protein by cell fusion method

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 21/00 (2006.01) C07H 21/04 (2006.01) C07K 14/00 (2006.01) C07K 16/00 (2006.01) C07K 16/24 (2006.01) C12N 5/12 (2006.01) C12N 5/16 (2006.01) C12N 5/26 (2006.01) C12N 15/00 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2268143

The present invention features a method of producing a multimeric protein from a hybrid cell formed from the fusion of two or more cells, each of which cell is engineered to express one component of the multimeric protein, as well as a method for screening for successful fusion of the cells to produce a desired hybrid cell. The methods of the invention are widely applicable to the production of proteins having two or more components.

Procédé de production d'une protéine multimère à partir d'une cellule hybride formée par fusion de deux ou plusieurs cellules, chacune de ces cellules étant recombinée par génie génétique pour exprimer un constituant de ladite protéine multimère, et procédé de criblage de la fusion réussie des cellules pour produire une cellule hybride désirée. Les procédés de la présente invention sont largement applicables à la production de protéines à deux ou plusieurs constituants.

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