Production of a soluble native form of recombinant protein...

Details Production of a soluble native form of recombinant protein... Production of a soluble native form of recombinant protein...

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01)

Patent

CA 2637881

The present invention is drawn to a method for enhancing secretional efficiency of a heterologous protein using a secretional enhancer consisting of a modified signal sequence which comprises the N-region of a signal sequence and/or a hydrophobic fragment of the said signal sequence comprising the said N-region and/or the hydrophilic polypeptide. The method of the present invention can be used not only for production of recombinant heterologous proteins by inhibiting insoluble precipitation and enhancing secretional efficiency of the recombinant protein into the periplasm or the extracellular fluid and but also for transduction of therapeutic proteins by enhancing membrane-permeability of the recombinant protein using a strong secretional enhancer.

L'invention concerne un procédé permettant de renforcer l'efficacité de sécrétion d'une protéine hétérologue au moyen d'un agent renforçant la sécrétion, ce dernier étant constitué d'une séquence signal modifiée comprenant la région N d'une séquence signal et/ou d'un fragment hydrophobe de ladite séquence signal comprenant ladite région N et/ou d'un polypeptide hydrophile. Le procédé de l'invention est utile non seulement pour produire des protéines hétérologues recombinées par l'inhibition d'un précipité insoluble et le renforcement de l'efficacité de sécrétion de la protéine recombinée dans le périplasme ou le fluide extracellulaire, mais aussi pour la transduction de protéines thérapeutiques par l'accroissement de la perméabilité membranaire de la protéine recombinée à l'aide d'un puissant agent renforçant la sécrétion.

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