Production of a viable, storable worm egg suspension

Details Production of a viable, storable worm egg suspension Production of a viable, storable worm egg suspension

A - Human Necessities

01

N

A01N 1/02 (2006.01) A61K 31/192 (2006.01) A61K 31/216 (2006.01) A61K 31/7028 (2006.01) A61K 35/00 (2006.01) A61P 1/00 (2006.01) A61P 37/02 (2006.01)

Patent

CA 2682525

The present application describes a novel, advantageous purification method which guarantees the embryonation capability and the viability of a worm egg suspension. The T. suis worm eggs are first treated in a non-embryonated condition in a sulfuric acid solution at about <= ph 2. Bacteria and viruses are thereby killed successfully. The germination number of yeasts and fungi is reduced. In a second step, the pH-value is increased and an orally tolerated preservative is added. Yeasts and fungi are thereby killed successfully. The suspension medium used in the second purification step can then be used as a culture medium for additional production (embryonation) and storage.

La présente invention concerne un nouveau procédé de purification avantageux, qui assure l'aptitude à la formation de l'embryon et la viabilité d'une suspension d'AEufs d'helminthes. A l'état non embryoné, les AEufs d'helminthes Trichuris suissont d'abord traités dans une solution d'acide sulfurique à un pH approximativement <= 2. Ainsi, les bactéries et virus sont efficacement éliminés. Le nombre de germes de levures et champignons est réduit. Dans une deuxième étape, le pH est augmenté et un agent de conservation compatible avec une administration orale est ajouté. Ainsi, les levures et champignons sont alors efficacement éliminés. Le milieu de suspension utilisé dans la deuxième étape de purification peut alors être utilisé comme milieu de culture pour d'autres préparations (formation d'embryons) et stockage.

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