Production of .alpha.-keto butyrate

Details Production of .alpha.-keto butyrate Production of .alpha.-keto butyrate

C - Chemistry, Metallurgy

12

P

C12P 7/40 (2006.01) C12P 17/04 (2006.01)

Patent

CA 2467965

A process of obtaining .alpha.-keto butyrate by bioconversion is disclosed. The molecule is accumulated in the medium and is apt to subsequent processing to food ingredients, such as top-note flavors. A suitable strain is a natural ilv-3 mutant Neurospora crassa strain, which is capable of accumulating .alpha.-keto butyrate in the fermentation medium: By adjusting specific medium parameters, up to 8 g/l medium of .alpha.-keto butyrate was accumulated.

L'invention concerne l'obtention d'.alpha.-cétobutyrate par bioconversion. La molécule est accumulée dans le milieu et elle est apte, après des traitements ultérieurs, à servir dans des ingrédients alimentaires, notamment dans des arômes de tête. Le mutant ilv-3 naturel de Neurospora crassa représente une souche adéquate, capable d'accumuler l'agr;-cétobutyrate dans le milieu de fermentation. Le réglage de paramètres spécifiques du milieu permet d'accumuler jusqu'à 8 g d'.alpha.-cétobutyrate par litre de milieu.

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