Production of antibodies using cre-mediated site-specific...

Details Production of antibodies using cre-mediated site-specific... Production of antibodies using cre-mediated site-specific...

C - Chemistry, Metallurgy

12

N

C12N 15/90 (2006.01) C07K 16/00 (2006.01) C07K 16/18 (2006.01) C12N 5/10 (2006.01) C12N 5/12 (2006.01) C12N 15/13 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2216609

A method to produce a cell expressing an antibody from a genomic sequence of a cell which has a modified immunoglobulin locus is disclosed. The method takes advantage of Cre-mediated site-specific recombination and homologous recombination. The method involves first introducing a lox site into an immunoglobulin locus and then using the lox site as a target for introduction of gene modifications via Cre-mediated in vivo recombination.

Méthode de production d'une cellule exprimant un anticorps à partir d'une séquence génomique d'une cellule comprenant un locus d'immunoglobuline modifiée. Le procédé consiste à exploiter la recombinaison spécifique à un site par Cre ainsi que la recombinaison homologue. Il consiste à d'abord introduire un site lox dans un locus d'immunoglobuline et à utiliser ledit site comme cible pour l'introduction de modifications géniques par recombinaison in vivo induite par Cre.

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