C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) A01K 67/027 (2006.01) C12N 5/02 (2006.01) C12N 5/06 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01)
Patent
CA 2300336
A culture system for producing PGCs or EG cells by culturing PGCs for long periods in tissue culture is provided. This culture system uses LIF, bFGF, IGF and SCF. The resultant EG cells are useful for the production of transgenic and chimeric avians, in particular, chickens and turkeys, and also for cloning purposes.
L'invention concerne un système de culture permettant d'élaborer des cellules sexuelles primordiales ou des cellules germinales d'embryon par la mise en culture tissulaire prolongée de cellules sexuelles primordiales. Le système de culture considéré fait appel aux facteurs suivants : facteur inhibiteur des leucocytes (LIF), facteur basique de croissance des fibroblastes (bFGF), facteur de croissance insuline-like (IGF) et facteur des cellules souches (SCF). Les cellules germinales d'embryon résultantes sont utiles pour la production d'oiseaux transgéniques et chimères (en particulier, poulets et dindes) ainsi que pour le clonage.
Jerry D. Joseph
Ponce de Leon F. Abel
Robl James M.
Stice Steven L.
Gowling Lafleur Henderson Llp
University Of Massachusetts A. Public Institution Of Higher Educ As Represented By Its Amherst Campus
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