Production of biologically active insulin-like growth factor...

Details Production of biologically active insulin-like growth factor... Production of biologically active insulin-like growth factor...

C - Chemistry, Metallurgy

12

P

530/15.04, 195/1

C12P 21/02 (2006.01) C07K 14/65 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2023969

Abstract "PRODUCTION OF BIOLOGICALLY ACTIVE INSULIN-LIKE GROWTH FACTOR I FROM HIGH EXPRESSION HOST CELL SYSTEMS" A novel method for refolding reduced IGF-I is provided in accordance with the present invention. The method is carried out by providing additional positive charge at the amino terminal end of the IGF-I molecule by the presence of a short leader sequence. The additional positive charge on the met-end of the IGF-I molecule has been found to enable recombinant IGF-I to refold simply by stirring solubilized inclusion body protein for between 2-16 hours, or overnight. The present invention also provides a novel fusion protein intermediate of IGF-I attached to the positively charged leader sequence.

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