Production of biologically active recombinant neurotrophic...

Details Production of biologically active recombinant neurotrophic... Production of biologically active recombinant neurotrophic...

C - Chemistry, Metallurgy

12

N

C12N 15/18 (2006.01) C07K 1/113 (2006.01) C07K 14/475 (2006.01) C07K 14/48 (2006.01)

Patent

CA 2189659

A process for the production of biologically active recombinant neurotrophic factor from the NGF/BDNF family is described. The process is comprised of: a) constructing a synthetic neurotrophic factor gene suitable for expression in a bacterial expression system, b) the synthetic neurotrophic factor gene is expressed in a bacterial expression system; c) the neurotrophic factor is solubilized and sulfonylated; d) sulfonylated neurotrophic factor is allowed to refold in the presence of polyethylene glycol and urea; and e) biologically active neurotrophic factor is isolated and purified.

L'invention concerne un procédé pour la production d'un facteur neurotrophique de recombinaison, biologiquement actif, à partir de la famille NGF/BDNF. Ce procédé consiste à a) construire un gène de facteur neurotrophique de synthèse pouvant être utilisé pour l'expression dans un système d'expression bactérien; b) assurer l'expression de ce gène de facteur neurotrophique de synthèse dans un système bactérien; c) solubiliser et sulfonylater ce facteur neurotrophique; d) permettre le repliement du facteur neurotrophique sulfonylaté en présence de polyéthylène glycol et d'urée; et e) isoler et purifier le facteur neurotrophique biologiquement actif.

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