Production of biomedical peptides and proteins in plants...

A - Human Necessities – 01 – H

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A01H 1/00 (2006.01) A61K 39/12 (2006.01) A61K 39/395 (2006.01) C07H 21/04 (2006.01) C07K 14/005 (2006.01) C07K 16/00 (2006.01) C07K 16/30 (2006.01) C12N 5/14 (2006.01) C12N 15/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2329074

The present invention discloses novel methods for producing foreign polypeptides in a host plant using recombinant viruses through functional complementation systems. The methods involve constructing suitable recombinant viral vectors that are capable of systemic infection and infecting the host plants with one or more recombinant viral vectors. The methods are also directed to infecting a host plant that is transgenic for expressing replicase genes of a virus, wherein the transgenic plant expressing a virus replicase genes complements the virus replicase function. The invention also discloses a method for producing a full-length antibody in a host plant using viral vectors.

La présente invention concerne de nouveaux procédés de production de polypeptides exogènes dans une plante hôte au moyen de virus de recombinaison par des systèmes de complémentation fonctionnelle. Les procédés consistent à construire des vecteurs viraux de recombinaison appropriés qui sont capables de produire une infection systémique et d'infecter les plantes hôtes avec un ou plusieurs vecteurs viraux de recombinaison. Les procédés visent également à infecter une plante hôte qui est transgénique pour exprimer les gènes réplicase d'un virus, la plante transgénique exprimant les gènes réplicase de virus complémentant la fonction réplicase du virus. L'invention concerne également un procédé de production d'un anticorps entier dans une plante hôte au moyen de vecteurs viraux.

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