Production of cell suspensions

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/00 (2006.01) A01N 63/00 (2006.01) C12N 5/02 (2006.01) C12N 5/06 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2472019

Methods are provided for producing cell suspensions suitable for engraftment and cell products suitable for infusion into a human patient. Some methods include (a) culturing a first population of cells that contains multiple cell types, including a first sub-population of cells of a type suitable for engraftment, under conditions that cause the expansion of the first sub- population of cells, wherein the population of cells further comprises a second sub-population of cells that either inhibit the expansion, or gwo less well under the culture conditions than the cells of the first sub-population, and that are capable of enhancing engraftment; (b) before, during, or following the culturing of step (a), removing from the population cells of the second sub-population; (c) preserving cells of the second sub-population; and (d) following expansion of the cells of the first sub-population, combining cells fo the first sub-population with cells of the preserved second sub- population.

La présente invention concerne des procédés pour produire des suspensions cellulaires adaptées à une prise de greffe et des produits cellulaires adaptés à une perfusion chez un patient humain. Certains procédés consistent (a) à mettre en culture une première population de cellules qui renferme plusieurs types de cellules, y compris une première sous-population de cellules d'un type adapté à la prise de greffe, dans des conditions induisant l'expansion de la première sous-population de cellules, la population de cellules renfermant également une seconde sous-population de cellules qui inhibent l'expansion ou qui se développent moins bien dans lesdites conditions de culture que les cellules de la première sous-population et qui peuvent améliorer la prise de greffe, (b) avant, pendant ou après la mise en culture de l'étape (a), à retirer de la population des cellules de la seconde sous-population, (c) à conserver des cellules de la seconde sous-population, puis (d), après l'expansion des cellules de la première sous-population, à combiner des cellules de la première sous-population et des cellules de la seconde sous-population conservées.

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