Production of cellulase enzymes in plant hosts using...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/42 (2006.01) C12N 15/00 (2006.01) C12N 15/84 (2006.01) C12N 15/56 (2006.01)

Patent

CA 2731866

Described herein are methods useful for producing proteins, such as enzymes, by agrofiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a cellulase, infecting plant cells with the Agrobacterium, allowing expression of the cellulase, and recovering the cellulase from the plant cells. In one embodiment, the protein produced is an endoglucanase.

L'invention concerne des procédés utiles pour la production de protéines, telles que des enzymes, par agro-filtration. Les procédés consistent à produire un Agrobacterium avec un plasmide Ti codant pour une cellulase, à infecter des cellules végétales avec Agrobacterium, à permettre une expression de la cellulase et à récupérer la cellulase des cellules végétales. Dans un mode de réalisation, la protéine produite est une endoglucanase.

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