C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) C12N 5/02 (2006.01) C12N 9/24 (2006.01) C12N 9/96 (2006.01)
Patent
CA 2099876
A method is provided for the production of biologically active glucocerebrosidase (GCR) in a cell culture. In one embodi- ment, recombinant cells containing the DNA sequence encoding for the production of GCR are cultured in a growth medium suitable for the enhancement of cell growth. A substantial portion of the growth medium may then be replaced with a production medium that is suitable for cell viability and secretion and stabilization of GCR. After production and secretion of GCR into the production medium, the production medium may be removed and collected. The GCR may then be purified from the collected production medium. Compositions, that are not toxic to the cells, may be added to the production medium to stabilize the secret- ed GCR. Stabilizing compositions that may be added include thiol-containing compositions such as dithiothreitol, glutathione, 1,3-mercaptoglycerol, cysteamine, lipoic acid, lipoamide and thioglycolate or proteins such as serum or serum albumin. In addi- tion, the pH or dissolved oxygen concentration of the production medium may be adjusted, or a combination of conditions may be utilized.
Barngrover Debra A.
Hayes Michael L.
Rasmussen James R.
Genzyme Corporation
Gowling Lafleur Henderson Llp
LandOfFree
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