Production of fibroblast growth factor

Details Production of fibroblast growth factor Production of fibroblast growth factor

C - Chemistry, Metallurgy

12

N

195/78, 195/1.2,

C12N 15/70 (2006.01) C07K 14/50 (2006.01) C12N 15/18 (2006.01)

Patent

CA 1323317

ABSTRACT "PRODUCTION OF FIBROBLAST GROWTH FACTOR" Disclosed is a process for producing E. coli derived recombinant fibroblast growth factor possessing part or all of the primary structural conformation and one or more of the biological properties of a mammalian (e.g., human) basic fibroblast growth factor which process is characterized as the host expression of an exogenous DNA sequence. Sequences coding for part or all of the sequence of amino acid residues of bFGF may be incorporated in autonomously replicating plasmid or viral vectors having a regulated promoter sequence and a temperature inducible copy number control gene employed to transform suitable procaryotic host cells such as E. coli in culture.

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