Production of fucosylated carbohydrates by enzymatic...

Details Production of fucosylated carbohydrates by enzymatic... Production of fucosylated carbohydrates by enzymatic...

C - Chemistry, Metallurgy

12

P

C12P 19/44 (2006.01) C07H 3/06 (2006.01) C07H 5/00 (2006.01) C07H 5/02 (2006.01) C07H 11/04 (2006.01) C07H 13/02 (2006.01) C07H 13/04 (2006.01) C07H 13/08 (2006.01) C07H 15/18 (2006.01) C07H 19/20 (2006.01) C07H 23/00 (2006.01) C12N 9/10 (2006.01) C12P 19/18 (2006.01) C12P 19/26 (2006.01)

Patent

CA 2121365

This invention-contemplates improved methods of enzymatic production of carbohydrates especially fucosylated carbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using both chemical and enzymatic means are also contemplated. The phosphorylated glycosides are then used to produce sugar nucleotides that are in turn used as donor sugars for glycosylation of acceptor carbohydrates. Especially preferred herein in the use of a disclosed method for fucosylation.

L'invention se rapporte à des procédés de production enzymatique d'hydrates de carbone, en particulier des hydrates de carbone fucosylés. Des synthèses améliorées de 1- ou 2-phosphates de glycosyle, effectuées à la fois par l'intermédiaire de moyens enzymatiques et de moyens chimiques sont également décrits. Les glucosides phosphorylés sont ensuite utilisés pour produire des nucléotides de sucres qui sont à leur tour utilisés comme sucres donneurs pour la glycosylation d'hydrates de carbone accepteurs. L'utilisation d'un procédé de fucosylation décrit est particulièrement préférée.

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