Production of galactosylated glycoproteins in lower eukaryotes

C - Chemistry – Metallurgy – 12 – P

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C12P 21/06 (2006.01) C12N 1/18 (2006.01)

Patent

CA 2562772

The present invention provides a novel lower eukaryotic host cell producing human-like glycoproteins characterized as having a terminal .beta.-galactose residue and essentially lacking fucose and sialic acid residues. The present invention also provides a method for catalyzing the transfer of a galactose residue from UDP-galactose onto an acceptor substrate in a recombinant lower eukaryotic host cell, which can be used as a therapeutic glycoprotein.

L'invention concerne une nouvelle cellule hôte eucaryote inférieure produisant des glycoprotéines humaines caractérisées en ce qu'elle possèdent un résidu .beta.-galactose terminal et qu'elles sont sensiblement exemptes de résidus fucose et acide sialique. L'invention porte également sur un procédé de catalyse du transfert d'un résidu UDP-galactose vers un substrat receveur dans une cellule hôte eucaryote inférieure de recombinaison, pouvant être utilisée en tant que glycoprotéine thérapeutique.

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