Production of glucagon like peptide 2 and analogs

Details Production of glucagon like peptide 2 and analogs Production of glucagon like peptide 2 and analogs

C - Chemistry, Metallurgy

12

N

C12N 15/16 (2006.01) A61K 38/26 (2006.01) C07K 14/605 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2546319

GLP-2 peptides and analogs thereof are produced in high yield and with desired, athentic termini by isolation from a GLP-2 peptide multimer in which at least two units of GLP-2 peptide are coupled through a linker that presents an N-terminal acid cleavage site and a C-terminal enzymr cleavage site. In a specific embodiment, [Gly2]hGLP-2 is produced from a multimeric precursor comprising 2-30 units thereof.

Les peptides GLP-2 et leurs analogues sont produits à haut rendement et avec des terminaisons authentiques désirées, par isolement à partir d'un multimère du peptide GLP-2 dans lequel au moins deux unités du peptide GLP-2 sont couplées par un lieur présentant un site de restriction de l'acide N-terminal et un site de restriction de l'enzyme C-terminale. Dans un mode de réalisation spécifique, [Gly2]hGLP-2 est produit à partir d'un précurseur multimère comprenant 2 à 30 unités du peptide.

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