Production of glucose from starch using alpha-amylases from...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/14 (2006.01) C11D 3/386 (2006.01) C11D 7/42 (2006.01) C12P 19/02 (2006.01) D06M 16/00 (2006.01) C12N 9/28 (2006.01) C12P 7/06 (2006.01)

Patent

CA 2726630

An alpha amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.

Une alpha-amylase provenant de Bacillus subtilis (AmyE) produit des quantités significatives de glucose à partir de divers substrats glucidiques, comprenant l'amidon végétal, le maltoheptaose et le maltotriose. Entre autres choses, cette propriété avantageuse permet à l'AmyE ou à ses variants d'être utilisés dans une réaction de saccharification ayant une exigence réduite ou supprimée pour une glucoamylase. La réduction ou la suppression de l'exigence de glucoamylase permet d'améliorer de façon significative le rendement de la production d'éthanol ou de sirop de maïs à teneur élevée en fructose, par exemple.

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