C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/139, 195/140
C12N 15/56 (2006.01) A23K 1/165 (2006.01) C12N 1/21 (2006.01) C12N 9/26 (2006.01) C12N 9/28 (2006.01) C12N 9/40 (2006.01) C12N 15/75 (2006.01) C12N 15/81 (2006.01) C12N 15/82 (2006.01) C12P 19/04 (2006.01) C12P 19/14 (2006.01)
Patent
CA 1339101
The gene encoding an alpha-galactosidase from guar seed has been isolated and identified. It has been expressed in yeasts, e.g. Saccharomyces, Xluyveromyces and Hansenula, in bacteria, e.g. Bacillus, and in plants, e.g. Nicotiana. The resulting alpha-galactosidase is able to decrease the galactose content of galactomannans such as guar gum by splitting off 1-6 linked alpha-D-galactopyranosyl units attached to a main chain of 1-4 linked beta-mannopyranosyl units. Thus a commercially feasible process becomes available for producing alpha-galactosidases suitable for providing galactomannans having improved properties, which can meet the increasing demand for good quality polysaccharides. It has further been demonstrated that other alpha-galactosidases, which show a positive immunological cross-reaction with an antibody raised against the alpha-galactosidase from guar, are also effective in decreasing the galactose content of galactomannans such as guar.
538543
Fellinger Arthur Johannes
Hughes Stephen Glyn
Overbeeke Nicolaas
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Plc Unilever
Quest International Services B.v.
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