Production of high mannose proteins in plant culture

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) A61K 38/47 (2006.01) C07K 19/00 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01) C12N 15/56 (2006.01) C12N 15/82 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2685400

A device, system and method for producing glycosylated proteins in plant culture, particularly proteins having a high mannose glycosylation, while targeting such proteins with an ER signal and/or by-passing the Golgi. The invention further relates to vectors and methods for expression and production of enzymatically active high mannose lysosomal enzymes using transgenic plant root, particularly carrot cells. More particularly, the invention relates to host cells, particularly transgenic suspended carrot cells, vectors and methods for high yield expression and production of biologically active high mannose Glucocerebrosidase (GCD). The invention further provides for compositions and methods for the treatment of lysosomal storage diseases.

L'invention concerne un dispositif, un système et un procédé pour la production de protéines glycosylées dans la culture de plantes, notamment de protéines présentant une teneur élevée en glycosylation du mannose, en ciblant de telles protéines qui ont un signal ER et/ou qui contournent l'appareil de Golgi. L'invention concerne également des vecteurs et des procédés pour l'expression et la production d'enzymes lysosomales à teneur élevée en mannose et actives enzymatiquement en utilisant la racine de plantes transgéniques, notamment de cellules de carotte. L'invention concerne plus particulièrement des cellules hôtes, notamment des cellules de carotte transgéniques en suspension, des vecteurs et des procédés d'expression à haut rendement et de production de glucocérébrosidase (GCD) à teneur élevée en mannose et active biologiquement. L'invention concerne également des compositions et des procédés de traitement de maladies du stockage lysosomal.

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