Production of high titer helper-free retroviruses by...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 7/00 (2006.01) A61K 35/12 (2006.01) A61K 39/00 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 15/86 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2156725

A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.

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