Production of host cells containing mutiple integrating...

Details Production of host cells containing mutiple integrating... Production of host cells containing mutiple integrating...

C - Chemistry, Metallurgy

12

N

C12N 15/90 (2006.01) C07K 16/00 (2006.01) C12N 5/10 (2006.01) C12N 15/13 (2006.01) C12N 15/48 (2006.01) C12N 15/86 (2006.01) C12N 15/867 (2006.01) C12P 21/02 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2553989

The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector comprising an exogenous gene and methods of making such host cells by serial transduction or transfection. The present invention further provides methods of expressing increased levels of protein in host cells using such vectors.

L'invention concerne la production de protéines dans des cellules hôtes, et plus particulièrement, des cellules hôtes contenant de multiples copies intégrées d'un vecteur d'intégration qui comprend un gène exogène, et des procédés de production de ces cellules hôtes par transduction ou transfection sérielle. L'invention concerne également des méthodes permettant d'exprimer des niveaux accrus de protéine dans des cellules hôtes, telles que lesdits vecteurs

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