C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) C07K 14/505 (2006.01) C12N 5/16 (2006.01)
Patent
CA 2342859
The present invention relates to the production of the recombinant human erythropoietin in mammalian cells, particularly in kidney cells, transformed with an expression vector containing the human erythropoietin gene driven by the myeloproliferative sacoma virus promoter. Further, this invention relates to the preparation of erythropoietin-producing cells using the tryptophan synthetase gene from E. coli as a selectable marker. The invention is also directed to an improved cell culture process using a serum-free and protein- free culture medium.
L'invention porte sur la production d'érythropoïétine humaine de recombinaison dans des cellules de mammifères, en particulier dans des cellules transformées par un vecteur d'expression contenant le gène de l'érythropoïétine humaine activé par le virus du sarcome myéloproliférant agissant comme promoteur. L'invention porte également sur la préparation de cellules productrices d'érythropoïétine en utilisant comme marqueur sélectionnable le gène de la tryptophane synthétase du E. coli. L'invention porte en outre sur un procédé amélioré de culture utilisant un milieu de culture exempts de sérum et de protéines.
Borden Ladner Gervais Llp
Cytos Biotechnology Ag
Solidago Ag
LandOfFree
Production of human erythropoietin does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Production of human erythropoietin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of human erythropoietin will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1420518