C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
167/37, 530/15.0
C12N 15/09 (2006.01) C07K 14/15 (2006.01) C12P 21/00 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01) G01N 33/569 (2006.01)
Patent
CA 1340878
Two regions of the gene for the HTLV-I envelope were expressed in Escherichia coli by use of the vector pJLAl6. One corresonds to the carboxy-terminal region of the major envelope protein p46 and the other corresponds to the transmembrane protein p2lE. Reactivity of the expressed protein with human sera was tested by Western blot procedure. Each of the sera tested that had been shown to contain anti-HTLV-I or anti-HTLV-II antibodies by ELISA assay recognized the bacterially synthesized envelope proteins. There was no reaction detected when control sera. were tested. This system is useful for large scale seroepidemiological surveys for this and related human retroviruses.
492997
Lautenberger James A.
Papas Takis S.
Samuel Kenneth
Wong-Staal Flossie
Gowling Lafleur Henderson Llp
U.s. Departm Ent Of Commerce The United States Of America Represented By The Secretary
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