C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/18 (2006.01) C07K 14/65 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 15/81 (2006.01)
Patent
CA 2090969
Insulin-like growth factor-1 (IGF-1), a naturally occurring, relatively short, single chain polypeptide, is prepared by grow- ing methylotrophic yeast transformants containing in their genome at least one copy of DNA encoding IGF-1, in operational lin- kage with DNA encoding a signal sequence, which is effective for directing secretion of proteins from the host cells and which also includes the proteolytic processing syte lys-arg and may include one or more glu-ala sequences. In preferred embodiments the signal sequence is the S. cerevisiae alpha mating factor pre-pro sequence. Expression of both the DNA encoding IGF-1 and the pre-pro signal sequence are regulated by a promoter region derived from a methanol responsive gene of a methylotrophic yeast. DNA constructs and recombinant methylotrophic yeast strains used for the expression and secretion of IGF-1 are also pro- vided. For preferred embodiments, protease deficient Pichia pastoris strains are provided.
Brierley Russell Arthur
Davis Geneva Ruth
Gleeson Martin A.
Holtz Gregory Clyde
Howard Bradley D.
Merck Sharp & Dohme Corp.
Smart & Biggar
The Salk Institute Biotechnology/industrial Associates Inc.
LandOfFree
Production of insulin-like growth factor-1 in methylotrophic... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Production of insulin-like growth factor-1 in methylotrophic..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of insulin-like growth factor-1 in methylotrophic... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1965093