Production of interferon gamma

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.18, 195/1.

C12N 15/23 (2006.01) C07K 14/57 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1304704

ABSTRACT Interferon .gamma. is produced in higly purified form (activity exceeding 108 units/ml-day) by CHO cells cotransformed by a first plasmid bearing the genomic human interferon gene under the control of the SV40 early promoter, and a second plasmid bearing a DHFR gene under similar control. Methotrexate selection yielded a clone which was a particularly efficient producer. Production of interferon .gamma. may also be facilitated by a Harvey sarcoma virus enhancer sequence.

480192

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