Production of metabolites by methanogens

C - Chemistry – Metallurgy – 12 – P

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C12P 19/02 (2006.01) C07B 59/00 (2006.01) C07C 229/02 (2006.01) C07D 207/16 (2006.01) C07H 3/02 (2006.01) C12P 13/04 (2006.01)

Patent

CA 2083253

ABSTRACT The present invention relates to the preparation of isotope labelled metabolites such as amino acids and carbohydrates by growing methanogenic bacteria on labelled acetate. Methano- genic bacteria useful for this purpose are acid tolerant, require acetate in addition to CO2 and H2 for growth, use essentially non-cyclic biosynthetic pathways, demonstrate a low level of carbon exchange between the carboxyl group of acetate and CO2, and have a low level of acetate synthesis from CO2 in the presence of exogeneous acetate.

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