C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/13 (2006.01) C07H 21/00 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2138009
2138009 9411507 PCTABS00032 The invention is directed to a method for producing a recombinant protein having a preselected binding affinity and specificity for a given antigen. Generally, the method combines spleen fragment culture for the in vitro somatic mutation of B cells in response to antigen, followed by preferential amplification of RNA encoding heavy and light chains of desired antibodies produced by those B cells. The amplified RNA is then converted to DNA and incorporated into expression vectors; transfected host cells then express the antibody chains or a single chain antibody having preselected characteristics. Also disclosed are generic degenerate primer pools for the efficient amplification of RNA encoding mouse IgG.
Biery Matthew C.
Decker Debra J.
Gingeras Thomas R.
Klinman Norman R.
Linton Phyllis-Jean
Biery Matthew C.
Decker Debra J.
Gingeras Thomas R.
Klinman Norman R.
Linton Phyllis-Jean
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