Production of oil in microorganisms

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 7/64 (2006.01) C12M 1/00 (2006.01) C12N 1/00 (2006.01) C12N 1/12 (2006.01) C12N 1/13 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/20 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12P 5/00 (2006.01)

Patent

CA 2689724

The invention provides methods and compositions useful for the production of oil, fuels, oleochemicals, and other compounds in microorgansims. In particular, the invention provides oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. The invention also provides microbial cells containing exogenous genes encoding, for example, a lipase, sucrose transporter, sucrose invertase, fructokinase, a polysaccharide- degrading enzyme, fatty acyl-ACP thioesterase, fatty acyl-CoA/aldehyde reductase, fatty acyl-CoA reductase, fatty aldehyde reductase, fatty aldehyde decarbonylase, and/or an acyl carrier protein. The invention also includes methods of manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel.

L'invention concerne des procédés et compositions utiles pour la production d'huile, de carburants, de produits oléochimiques, et d'autres composés dans des micro-organismes. En particulier, l'invention propose des micro-organismes portant de l'huile et des procédés de mise en culture à bas coût de tels micro-organismes. L'invention propose également des cellules microbiennes contenant des gènes exogènes codant, par exemple, une lipase, un transporteur de saccharose, un inverseur de saccharose, de la fructokinase, une enzyme dégradant le polysaccharide, une acyl-ACP thioestérase grasse, une acyl-CoA/aldéhyde réductase grasse, une acyl-CoA réductase grasse, une aldéhyde réductase grasse, une aldéhyde décarbonylase grasse et/ou une protéine porteuse d'acyle. L'invention comprend également des procédés de fabrication de carburants de transport tels que du diesel renouvelable, du biodiesel et du carburant pour avion renouvelable.

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