Production of oligosaccharides in transgenic plants

Details Production of oligosaccharides in transgenic plants Production of oligosaccharides in transgenic plants

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) A01H 5/00 (2006.01) A23K 1/14 (2006.01) A23L 1/09 (2006.01) A23L 1/307 (2006.01) C07H 3/06 (2006.01) C08B 37/00 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2194579

The invention relates to a method for producing oligosaccharides, comprising of selecting a gene which codes for an enzyme which is capable of converting sucrose into an oligosaccharide; linking the gene to suitable transcription- initiation and transcription-termination signals in order to provide an expression construct; transforming a suitable plant cell with the expression construct; regenerating a transgenic plant from the transformed plant cell; culturing the transgenic plant under conditions enabling the expression and activity of the enzyme; and isolating the oligosaccharides from the transgenic plant. The invention further relates to the product obtained by means of the method and to the use thereof, in addition to transgenic plants and parts thereof which are capable of producing oligosaccharides.

Procédé de production d'oligosaccharides, consistant à sélectionner un gène codant une enzyme pouvant convertir le sucrose en un oligosaccharide; à lier le gène à des signaux appropriés d'initiation de transcription et de terminaison de transcription, afin de former un produit d'assemblage d'expression; à transformer une cellule végétale appropriée avec ce produit d'assemblage d'expression; à régénérer une plante transgénique à partir de la cellule de plante transformée; à cultiver la plante transgénique dans des conditions permettant l'expression et l'activité de l'enzyme; et à isoler les oligosaccharides de la plante transgénique. L'invention se rapporte également au produit obtenu par ce procédé et à son utilisation, ainsi qu'aux plantes transgéniques et aux parties de ces plantes pouvant produire des oligosaccharides.

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