Production of peptides and proteins by accumulation in plant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/425 (2006.01) C07K 14/585 (2006.01) C12N 15/18 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2490348

A nucleic acid molecule is disclosed as containing a first nucleic acid sequence comprising a nucleotide sequence that encodes .gamma.-zein protein, or a fragment thereof capable of directing and retaining a protein towards the endoplasmic reticulum (ER) of a plant cell; a second nucleic acid sequence containing a nucleotide sequence that encodes an amino acid sequence that is specifically cleavable by enzymatic or chemical means; and a third nucleic acid sequence containing the nucleotide sequence that encodes a peptide or protein of interest. Methods of using this nucleic acid molecule for transforming host plant cells and producing the peptide or protein of interest are also disclosed.

L'invention concerne une molécule d'acide nucléique contenant une première séquence d'acides nucléiques comprenant une séquence nucléotidique codant pour la protéine .gamma.-zéine, ou un fragment de celle-ci pouvant diriger et retenir une protéine vers le réticulum endoplasmique (ER) d'une cellule végétale; une deuxième séquence d'acides nucléiques contenant une séquence nucléotidique codant pour une séquence d'acides aminés pouvant être coupés de manière spécifique par des moyens enzymatiques ou chimiques; et une troisième séquence d'acides nucléiques contenant la séquence nucléotidique codant pour un peptide ou une protéine d'intérêt. L'invention concerne également des méthodes d'utilisation de cette molécule d'acide nucléique pour transformer des cellules végétales hôtes et produire le peptide ou la protéine d'intérêt.

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