Production of phosphopeptides from casein

Details Production of phosphopeptides from casein Production of phosphopeptides from casein

C - Chemistry, Metallurgy

12

P

C12P 21/06 (2006.01) A23J 3/10 (2006.01) A23J 3/34 (2006.01) C07K 1/14 (2006.01) C07K 7/08 (2006.01) C07K 14/47 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2108678

A method for the preparation of selected phosphopeptides having anticariogenic and other activities, comprising the steps of completely digesting a soluble monovalent ration salt of casein in solution with a proteolytic enzyme, adding a mineral acid to the solution to adjust the pH to about 4.7, removing any precipitate produced, adding CaC1 2 to a level of about 1.0 % w/v to cause aggregation of at least the selected phosphopeptides in said digested solution, separating the aggregated phosphopep- tides from the solution through a filter having a molecular weight exclusion limit lying substantially within the range 10,000 to 20,000 while passing the bulk of the remaining phosphopeptides and solution, diafiltering the separated phosphopeptides with water through a filter and concentrating and drying the retentate.

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