C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/47, 195/86
C12N 5/04 (2006.01) A01H 4/00 (2006.01) C12N 5/00 (2006.01) C12P 1/00 (2006.01) C12P 17/18 (2006.01)
Patent
CA 2028523
PRODUCTION OF PODOPHYLLOTOXINS USING PODOPHYLLUM ABSTRACT OF THE DISCLOSURE A process for the preparation of an adventive embryo of a plant belonging to the genus Podophyllum, comprising the steps of preparing cells of a plant belonging to the genus Podophyllum, incubating the cells in a sugar free medium or a medium containing up to 2% by weight sugar, and then incubating the cells in a medium containing more than 2% by weight of sugar to generate an adventive embryo; a process for the production of a podophyllotoxin compound comprising the steps of preparing an adventive embryo of a plant belonging to the genus Podophyllum, culturing the adventive embryo to generate an adventitious organ, culturing the adventitious organ to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound; and a process for the production of a podophyllotoxin compound, comprising the steps of preparing de-differentiated cells or tissue of a plant belonging to the genus Podophyllum culturing the de-differentiated cells or tissue in a liquid medium to generate a differentiated organ, culturing the differentiated organ on a solid medium to produce a podophyllotoxin compound, and recovering the podophytlotoxin compound.
Morita Eitaroh
Sakata Ko
Takezono Tetsuya
Fetherstonhaugh & Co.
Nippon Oil Co. Ltd.
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