Production of polyketide fkbp-ligand analogues

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 1/21 (2006.01) A61K 31/436 (2006.01) C07D 498/18 (2006.01) C12N 15/52 (2006.01) C12N 15/70 (2006.01) C12P 1/04 (2006.01) C12P 17/18 (2006.01) C12P 19/62 (2006.01)

Patent

CA 2492153

The present invention relates to production of polyketides and other natural products and to libraries of compounds and individual novel compounds. One important area is the isolation and potential use of novel FKBP-ligand analogues and host cells that produce these compounds. The invention is particularly concerned with methods for the efficient transformation of strains that produce FKBP analogues and recombinant cells in which cloned genes or gene cassettes are expressed to generate novel compounds such as polyketide (especially rapamycin) FKBP-ligand analogues, and to processes for their preparation, and to means employed therein (e.g. nucleic acids, vectors, gene cassettes and genetically modified strains).

la pr~sente invention concerne la production de polyk~tides et autres produits naturels ainsi que des banques de compos~s et des compos~s individuels nouveaux. Un aspect important concerne l'isolement et l'utilisation potentielle de nouveaux analogues de ligand FKBP et des cellules hÙtes qui produisent ces compos~s. L'invention concerne en particulier des m~thodes de transformation efficace de souches produisant des analogues de FKBP et des cellules de recombinaison dans lesquelles des g­nes clon~s ou des cassettes de g­nes sont exprim~s et produisent des nouveaux compos~s tels que des analogues de ligand polyketidiques (en particulier la rapamycine) et leurs m~thodes de pr~paration et moyens utilis~s (tels qu'acides nucl~iques, vecteurs, cassettes de g­nes et souches g~n~tiquement modifi~es).

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